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Figure 2 | BMC Biotechnology

Figure 2

From: Disruption of Trichoderma reesei cre2, encoding an ubiquitin C-terminal hydrolase, results in increased cellulase activity

Figure 2

Complementation of A. nidulans creB1937 by T. reesei cre2. The A. nidulans creB1937 mutant strain was co-transformed with pPL3 and pTRcre2 containing Trichoderma reesei cre2, and six riboflavine independent transformants, T1-T6, were tested for complementation of creB1937 phenotypes. Strains were incubated at 37°C on a) 1% glucose, 10 mM ammonium tartrate; b) 1% glucose, 10 mM ammonium tartrate, 10 mM allyl alcohol; c) key to strains. Plates were supplemented with riboflavin and para amino benzoic acid.

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