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Table 1 Primers used for STR amplification

From: Authentication of African green monkey cell lines using human short tandem repeat markers

STR Marker

Forward Primer (5'-3')

Reverse Primer (5'-3')

Primer μM

D8S1106

[FAM]-GTTTACCCCTGCATCACTGG

GTCAGAATTGCTCATAGTGCAAGA

0.045

DYS389

[FAM]-CCAACTCTCATCTGTATTATCTATG

GTCTTATCTCCACCCACCAGA

0.200

D1S518

[VIC]-GCAGATCTTGGGACTTCTCG

GTGTGGGCAACTGCATTAGAG

0.420

D6S1017

[VIC]-CTGGCACAGGATAGGTGCTC

GATTGAACCAGATGGGAACGA

0.300

D17S1304

[NED]-ACCATGTCCTCTGGTTCTGG

GTTTCTTACAGGTGGGACTTGGTGAAA

0.040

D4S2408

[NED]-TCATTTCCATAGGGTAAGTGAAAA

GCCATGGGGATAAAATCAGA

0.200

D5S1467

[PET]-GCCTAAGGTGGTGAATTGGA

GTGCATTATTGGAGGCTTTCTC

0.060

D19S245

[PET]-GACCTGCAATCAGCCATTTT

GTTCTTGCAGTCTGTGGCTTG

0.360

  1. Reverse primers that did not end in a guanine base at the 5'end were modified by adding an additional guanine (G) base or a "PIGtail" sequence (GTTTCTT) to the 5' end of the reverse primer (underlined) to promote complete adenylation [37]. Primer concentrations listed are final concentrations of forward and reverse primers in a 20 μL reaction volume. Primer concentrations were determined empirically based on peak height, DNA concentration, and number of cycles in the PCR program.