Figure 2

Electropherogram representing a mixture of Vero and HeLa cell DNA (10:1 ratio, respectively). Genetic profiles were analysed using bins and panels developed in GeneMapper ID-X. Relative fluorescent units (RFUs) are depicted on the y-axis and fragment length on the x-axis in the electropherogram. Arrows depict HeLa alleles which may or may not appear in the bins and panels designated for Vero samples. Vero alleles appear in the shaded bins and panels designated for each STR marker. The number(s) below each peak represent the number of repeats at that locus. At the D4S2408, D5S4167, and D19S245 markers, HeLa DNA amplifies outside the observed vervet allele range and those values are represented as fragment lengths (bp) instead of repeat numbers. HeLa cells do not amplify at the DYS389 locus because they are of female origin. Human allele ranges that differ from vervet monkeys are illustrated in yellow boxes.