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Table 3 Parameters of NOX immobilization onto agarose via different chemistries.

From: New biotechnological perspectives of a NADH oxidase variant from Thermus thermophilus HB27 as NAD+-recycling enzyme

Activated agarosea

Immobilization yield

Ψ (%)b

Expressed activity

Ae (%)c

Gx-ag a

85

60

CNBr-ag a

100

80

IDA-Cu 2+ -ag a

100

80

  1. a 6BCL agarose was activated with different functional groups as methods described in order to immobilized NOX through different chemistries, giving each one different properties to the final insoluble preparation. b Immobilization yield was calculated as follows; Ψ (%) = (Supernatant activity after incubation with the support/Supernantant activity before incubation with the support)*100.c Expressed activity was calculated; Ae (%) = (Activity/g of support)/(Immobilized activity/g of support)*100.
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BMC Biotechnology

ISSN: 1472-6750

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