|
Activated agarosea
|
Immobilization yield
Ψ (%)b
|
Expressed activity
Ae (%)c
|
|---|
|
Gx-ag
a
|
85
|
60
|
|
CNBr-ag
a
|
100
|
80
|
|
IDA-Cu
2+
-ag
a
|
100
|
80
|
- a 6BCL agarose was activated with different functional groups as methods described in order to immobilized NOX through different chemistries, giving each one different properties to the final insoluble preparation. b Immobilization yield was calculated as follows; Ψ (%) = (Supernatant activity after incubation with the support/Supernantant activity before incubation with the support)*100.c Expressed activity was calculated; Ae (%) = (Activity/g of support)/(Immobilized activity/g of support)*100.
