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Table 2 Kinetics parameters of NOX towards different cofactors.

From: New biotechnological perspectives of a NADH oxidase variant from Thermus thermophilus HB27 as NAD+-recycling enzyme

 

Km

(mM)

kcat

(s-1)

kcat/Km

× 10 6 (M-1*s-1)

NADHa

2.1 ± 0.4

15.6 ± 0.7

7.4

FADb

34.1 ± 6.0

-

-

FMNb

42.8 ± 5.1

-

-

  1. The steady-state kinetics parameters were calculated at pH 7 and 25°C (See methods). Activities were adjusted to a non-linear regression.a Kinetics parameters for NADH were calculated using 50 μM FAD.b Kinetics parameters for flavin cofactors were calculated using 10 μM NADH.
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BMC Biotechnology

ISSN: 1472-6750

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