Table 1 Expression level and stability of anti-VLY scFvs
Anti-VLY scFv | Expression level, % of total cell protein | Oligomeric state | Activity (%) after 12 months storage at 4°C |
|---|---|---|---|
scFv 9B4 (VL-VH) | 20-30 | monomeric | 100 |
scFv 9B4 (VH-VL) | 20-30 | monomeric | 100 |
scFv 23A2 (VL-VH) | 20-30 | monomeric | 75 |
scFv 23A2 (VH-VL) | 20-30 | monomeric | 75 |