Table 3 Primers used for the construction of plasmids for T2 transcript luciferase assays.
Forward primer | Reverse primer | Vector name |
|---|---|---|
FKO13-NheI 5'-ccgggctagcgtttatttccaacagagtacacgactc-3' | FKO12-XhoI 5'-ccggctcgaggtttcctacccgctcgcactcg-3' | pGL3(-1233/-404) |
FKO15-NheI 5'-ccgggctagccaggtgaggagctgctgaaggcacagac-3' | FKO12-XhoI 5'-ccggctcgaggtttcctacccgctcgcactcg-3' | pGL3(-998/-404) |
FKO17-NheI 5'-ccgggctagcgtcttgagggcagcttttctactcagc-3' | FKO12-XhoI 5'-ccggctcgaggtttcctacccgctcgcactcg-3' | pGL3(-720/-404) |
FKO11-NheI 5'-ccgggctagcacattccacccctgactcctaagaccaac-3' | FKO14-XhoI 5'-ccggctcgaggtcgccggccgggtgccaccggggccaatc-3' | pGL3(-1399/-537) |
FKO13-NheI 5'-ccgggctagcgtttatttccaacagagtacacgactc-3' | FKO14-XhoI 5'-ccggctcgaggtcgccggccgggtgccaccggggccaatc-3' | pGL3(-1233/-537) |
FKO15-NheI 5'-ccgggctagccaggtgaggagctgctgaaggcacagac-3' | FKO14-XhoI 5'-ccggctcgaggtcgccggccgggtgccaccggggccaatc-3' | pGL3(-998/-537) |
FKO17-NheI 5'-ccgggctagcgtcttgagggcagcttttctactcagc-3' | FKO14-XhoI 5'-ccggctcgaggtcgccggccgggtgccaccggggccaatc-3' | pGL3(-720/-537) |