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Table 2 Reporter plasmids for T1 transcript luciferase assays.

From: Molecular cloning, characterization, genomic organization and promoter analysis of the α1,6-fucosyltransferase gene (fut8) expressed in the rat hybridoma cell line YB2/0

Digestion

Position of the fragment

Vector name

ApaI, HindIII

-1205/-411

pGL3(-1205/-411)

BseRI, HindIII

-1004/-411

pGL3(-1004/-411)

NheI, HindIII

-892/-411

pGL3(-892/-411)

MseI, HindIII

-598/-411

pGL3(-598/-411)

KpnI, PvuI

-1438/-451

pGL3(-1438/-451)

KpnI, PvuI

-1205/-451

pGL3(-1205/-451)

NheI, PvuI

-892/-451

pGL3(-892/-451)

KpnI, MseI

-1438/-598

pGL3(-1438/-598)

KpnI, MseI

-1205/-598

pGL3(-1205/-598)

NheI, MseI

-892/-598

pGL3(-892/-598)