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Table 1 Probes and primer sequences used for TaqMan™ quantitative PCR experiments

From: Molecular cloning, characterization, genomic organization and promoter analysis of the α1,6-fucosyltransferase gene (fut8) expressed in the rat hybridoma cell line YB2/0

Target

Forward Primer

Reverse Primer

Probe

Transcript T1

5'-cgcgggctgctgttc-3'

5'-ataataatggaaaggacttgatcttgg-3'

5'-ccctggtggcgtt-3'

Transcript T2

5'-ccgcttagctcgccctcta-3'

5'-aggactagatcttggatgaaaacg-3'

5'-agtccttcggcccacg-3'

HPRT

5'-tgtacttggcttttccactttcg-3'

5'-tgcccttgactataatgagcacttc-3'

5'-atgacacaaacatgattca-3'

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BMC Biotechnology

ISSN: 1472-6750

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