Figure 8

Luciferase assays after transfections in Rat2 cells of different plasmid constructs for T2 transcription analysis. (A) Location on the genomic sequence of the restriction sites used to generate the different deletions of the intronic region upstream exon E-3. Numbering begins with the initiation codon. (B) On the left, a schematic representation of the different constructs inserted in pGL3b upstream of the luciferase gene. The gray boxes represent the different deletions of the intronic region upstream exon E-3. The black box indicates the exon E-3. Luc indicates the Firefly luciferase coding sequence and the lines indicate the missing sequences. On the right: the results of luciferase assays. Transfection efficiencies were normalized with the co-transfected plasmid expressing Renilla luciferase and luciferase activities are expressed compared to pGL3b activity. The data are means +/- S.D. of n > = 3 experiments.