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Figure 4 | BMC Biotechnology

Figure 4

From: Molecular cloning, characterization, genomic organization and promoter analysis of the α1,6-fucosyltransferase gene (fut8) expressed in the rat hybridoma cell line YB2/0

Figure 4

RLM-RACE analysis of fut8 transcripts. (A) The nested PCR products obtained by RLM-RACE of fut8 transcripts were analyzed on 1.2% Agarose gel. 1: molecular ladder; 2: negative control of amplification; 3: PCR negative control; 4: nested PCR products. (B) Nucleotide sequence of 5'-untranslated regions of T1 [EMBL: FN668936], T2 [EMBL: FN668937] and T3 [EMBL: FN668938] transcripts. Numbering begins with the initiation codon. The beginning of the coding sequence is indicated in italics. Arrowheads indicate the different transcription start sites identified by RLM-RACE. The exon limits are indicated with back-to-back arrows.

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BMC Biotechnology

ISSN: 1472-6750

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