Comparative transfection of DNA into primary and transformed mammalian cells from different lineages

BMC Biotechnology

Table 1 Cells and Optimal Nucleofection Conditions

Species	Cell name	Cell description	AMAXA program	AMAXA buffer
Pig	P16	Pig Fetal Fibroblasts	U-20	NHDF
	PTE	Primary Pig Tracheal Epithelial Cells	T-20	Basic epithelial cell
Human	16HBE41o-	Immortalized Human Bronchial Epithelial cell Line (WT)	O-17	V
	CFBE41o-	Immortalized Human CF Bronchial Epithelial Cell Line (ΔF508/ΔF508))	O-17	V
	HTE	Primary Human Tracheal Epithelial Cells	T-20	Basic epithelial cell
	LT1-1B1	Immortalized Human Lymphoblasts (HPRT mutant)	G-16	T
	SC-1	Immortalized Human Lymphoblasts (β^S-globin mutant)	G-16	T
	HSPC	Primary Hematopoietic Stem/Progenitor Cells (CD34+ lin-)	U-08	CD34+
	HEK 293	Adenovirus immortalized human embryonic kidney cells	X-01	V
Rabbit	REF	Rabbit Ear Fibroblasts	U-23	NHDF
Mouse	MESC	Transgenic mouse embryonic stem cells (β^S-globin mutant)	A-24	Mouse ES cell

The table contains the cells used in the studies and their origin. For each cell line/type, the optimal nucleofection program and buffer are indicated.

ISSN: 1472-6750