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Table 1 Correlation of the multiplex endpoint RT-PCR and the RT-qPCR assay.

From: A multiplex endpoint RT-PCR assay for quality assessment of RNA extracted from formalin-fixed paraffin-embedded tissues

Multiplex RT-PCR Protocol No. samples in RT-PCR No. samples in RT-qPCR
No amplification P1 3/90 (3%) Cq < 35.1 : 0
Cq ≥ 35.1 : 3
  P2 7/90 (8%) Cq < 35.1 : 0
Cq ≥ 35.1 : 7
92 bp P1 13/90 (15%) Cq < 35.1 : 5
Cq ≥ 35.1 : 8
  P2 18/90 (20%) Cq < 35.1 : 10
Cq ≥ 35.1 : 8
>92 bp P1 74/90 (82%) Cq < 35.1 : 73
Cq ≥ 35.1 : 1
  P2 65/90 (72%) Cq < 35.1 : 62
Cq ≥ 35.1 : 3