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Table 1 Correlation of the multiplex endpoint RT-PCR and the RT-qPCR assay.

From: A multiplex endpoint RT-PCR assay for quality assessment of RNA extracted from formalin-fixed paraffin-embedded tissues

Multiplex RT-PCR

Protocol

No. samples in RT-PCR

No. samples in RT-qPCR

No amplification

P1

3/90 (3%)

Cq < 35.1 : 0

Cq ≥ 35.1 : 3

 

P2

7/90 (8%)

Cq < 35.1 : 0

Cq ≥ 35.1 : 7

92 bp

P1

13/90 (15%)

Cq < 35.1 : 5

Cq ≥ 35.1 : 8

 

P2

18/90 (20%)

Cq < 35.1 : 10

Cq ≥ 35.1 : 8

>92 bp

P1

74/90 (82%)

Cq < 35.1 : 73

Cq ≥ 35.1 : 1

 

P2

65/90 (72%)

Cq < 35.1 : 62

Cq ≥ 35.1 : 3