Multiplex RT-PCR | Protocol | No. samples in RT-PCR | No. samples in RT-qPCR |
---|---|---|---|
No amplification | P1 | 3/90 (3%) | Cq < 35.1 : 0 Cq ≥ 35.1 : 3 |
 | P2 | 7/90 (8%) | Cq < 35.1 : 0 Cq ≥ 35.1 : 7 |
92 bp | P1 | 13/90 (15%) | Cq < 35.1 : 5 Cq ≥ 35.1 : 8 |
 | P2 | 18/90 (20%) | Cq < 35.1 : 10 Cq ≥ 35.1 : 8 |
>92 bp | P1 | 74/90 (82%) | Cq < 35.1 : 73 Cq ≥ 35.1 : 1 |
 | P2 | 65/90 (72%) | Cq < 35.1 : 62 Cq ≥ 35.1 : 3 |