Comparison of the multiplex endpoint RT-PCR and RT-qPCR assays for protocol 1 (P1). The bottom axis identifies the samples. The band sizes obtained for each of the sample replicate are shown as bars. The values on the top of the graph provide both the Cq value measured for each sample replicate in the TBP RT-qPCR assay and the mean Cq value. Symbols are as follows: * indicates a standard deviation between replicates that is greater than 0.5; ** one out of two replicates amplified.