Figure 2

Primer placement for the multiplex endpoint RT-PCR and the RT-qPCR assay. Both assays use the TBP mRNA (NM_003194) as the target sequence. The eight exons are shown as rectangles labelled from E1 to E8. The primer locations are shown as horizontal arrow heads. The positions of the primers for the multiplex endpoint RT-PCR assay (92-F, 92-R, 161-F, 161-R, 252-F, 252-R, 300-F and 300-R) and for the RT-qPCR assay (RT-qPCR-F and RT-qPCR-R) are shown. Each primer pair was designed to span at least one intron.