Figure 1

Assessment of total RNA yield and purity extracted by the two different RNA extraction protocols. The x-axis shows the sample identification. The y-axis on the left shows the RNA concentration in ng/μL. The RNA concentrations for each of the sample replicates are shown as groups of three bars. The y-axis on the right shows the absorbance ratio A₂₆₀/A₂₈₀. The absorbance ratio A₂₆₀/A₂₈₀ for each of the sample replicates is shown as a scatter graph (■: replicate 1; ▲: replicate 2; ●: replicate 3). Panel A corresponds to protocol 1 (P1) and Panel B corresponds to protocol 2 (P2). Note that for the RNA concentration, panel A is scaled differently to panel B as the RNA extracted by the two different RNA extraction protocols was eluted in different final elution volumes.