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Table 2 Reactivity patterns of all generated mAbs with HEK cells and P. falciparum parasites

From: An efficient system to generate monoclonal antibodies against membrane-associated proteins by immunisation with antigen-expressing mammalian cells

  

Western blotting analysis

IFA

Hybridoma

Clone

Isotype

HEK

PFF0620c

HEK

blood

stages

sporozoites

HEK

PFF0620c

HEK

blood

stages

sporozoites

PFF0620c 01

IgG2a

-

+

-

-

-

+

-

+

PFF0620c 02

IgG2a

-

+

-

-

-

+

-

-

PFF0620c 06

IgG1

-

+

-

-

-

+

-

+

PFF0620c 07

IgG2a

-

+

-

-

-

+

-

+

PFF0620c 08

IgG1

-

+

-

-

-

+

-

-

PFF0620c 09

IgG1

-

+

-

-

-

+

-

+

PFF0620c 10

IgG1

-

+

-

-

-

+

-

+

PFF0620c 11

IgG1

-

+

-

-

-

+

-

+

PFF0620c 13

IgG2a

-

+

-

-

-

+

-

+

PFF0620c 17

IgG2b

-

+

-

-

-

+

-

+

PFF0620c 19

IgG1

-

+

-

-

-

+

-

-

PFF0620c 21

IgG2b

-

+

-

-

-

+

-

+

PFF0620c 22

IgG1

-

+

-

-

-

+

-

+

PFF0620c 25

IgG1

-

+

-

-

-

+

-

+

PFF0620c 27

IgG2b

-

+

-

-

-

+

-

-

PFF0620c 29

IgG1

-

+

-

-

-

+

-

+

PFF0620c 32

IgG1

-

-

-

-

-

+

-

+

  

0/17

16/17

0/17

0/17

0/17

17/17

0/17

13/17

Hybridoma

Clone

Isotype

HEK

PFD1130w

HEK

blood

stages

sporozoites

HEK

PFD1130w

HEK

blood

stages

sporozoites

PFD1130w 02

IgG2b

-

+

+

-

-

+

+

-

PFD1130w 04

IgG3

-

+

+

-

-

+

+

-

PFD1130w 05

IgG2a

-

+

+

-

-

+

+

-

PFD1130w 06

IgG2a

-

+

+

-

-

+

+

-

PFD1130w 08

IgG1

-

+

+

-

-

+

+

-

PFD1130w 09

IgG3

-

+

+

-

-

+

+

-

PFD1130w 10

IgG1

-

+

+

-

-

+

+

-

PFD1130w 12

IgG2a

-

+

+

-

-

+

+

-

PFD1130w 13

IgG1/IgG2b

-

+

+

-

-

+

+

-

  

0/9

9/9

9/9

0/9

0/9

9/9

9/9

0/9

Hybridoma

Clone

Isotype

HEK

PF14_0325

HEK

blood

stages

sporozoites

HEK

PF14_0325

HEK

blood

stages

sporozoites

PF14_0325 02

IgG1

-

+

+

nd

-

+

+

nd

PF14_0325 04

IgG1

-

+

+

nd

-

+

+

nd

  

0/2

2/2

2/2

 

0/2

2/2

2/2

 
  1. nd = no data