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Figure 7 | BMC Biotechnology

Figure 7

From: An efficient system to generate monoclonal antibodies against membrane-associated proteins by immunisation with antigen-expressing mammalian cells

Figure 7

Western blot analysis of generated monoclonal antibodies with the recombinant P. falciparum proteins. Specificity of representative monoclonal antibodies for the corresponding recombinant proteins is demonstrated by Western-blot analysis. Reduced (A) and non-reduced (B) protein lysates of PFF0620c- (line 1), PFD1130w- (line 2), PF14_0325-expressing HEK cells (line 3) and non-transfected HEK cells (line 4) were probed with anti-His tag mAb, anti-PFD1130w 02 mAb, anti-PF14_0325 04 mAb and anti-PFF0620c 07 mAb, respectively.

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