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Figure 1 | BMC Biotechnology

Figure 1

From: An efficient system to generate monoclonal antibodies against membrane-associated proteins by immunisation with antigen-expressing mammalian cells

Figure 1

Construction of expression plasmids. Schematic diagram of pcDNA3.1 BVM_PFF0620C_FLAG_GP_His, pcDNA3.1 BVM_PFD1130W_FLAG_GP_His and pcDNA3.1 BVM_PF14_0325_FLAG_GP_His (pcDNA3.1 BVM_P. falciparum ORF_FLAG_GP_His). The commercially available pcDNA3.1 expression plasmid (Invitrogen) was modified to contain chimeric genes consisting of the secretion signal sequence of bee venom melittin, the codon-optimised sequence encoding the P. falciparum proteins PFF0620C, PFD1130W or PF14_0325 without secretion signal sequence and GPI-attachment signal sequence, a FLAG tag encoding sequence, a sequence encoding the transmembrane domain of mouse glycophorin-A and a His tag encoding sequence.

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BMC Biotechnology

ISSN: 1472-6750

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