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Table 2 Application of Plackett-Burman design at various process parameters (designated by different captions) for L-dopa production by A. niger GCBT-8

From: Production of 3,4-dihydroxy L-phenylalanine by a newly isolated Aspergillus niger and parameter significance analysis by Plackett-Burman design

Process parameters at 2-factorial design

Tyrosinase activity (U/mg)

L-dopa production (mg/ml)

Time course (h)A

Initial pHB

Glucose conc. (%, w/v)C1+C2

Ammonium chloride conc. (%, w/v)D

Potassium dihydrogen phosphate conc. (%, w/v)E

 

Observed

Predicted

  

Intact

Grinded

     

24

4.5

2

4

-

0.1

16.82

0.124

0.196

24

5

4

4

-

0.2

28.06

0.171

0.215

48

5

4

6

0.2

0.3

43.28

0.228

0.266

48

5.5

6

6

0.2

0.3

64.55

0.365

0.388

72

6

6

8

0.4

0.4

49.34

0.302

0.356

  1. The different letters represent significant process parameters for L-dopa production. Statistical analysis of the model was based on 2-factorial experimental design. The dashes indicate the control experiments with no nutritional supplementation.