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Table 1 Sequences and modifications of deoxyoligonucleotides used in this study

From: DNAzyme-mediated recovery of small recombinant RNAs from a 5S rRNA-derived chimera expressed in Escherichia coli

Name of oligos

Sequence (from 5' to 3')

Length (nt)

Pen17zyme1

CGCTAATTGGTTA TCCGAGCCGGTCGAA CGTAACATGAGTT

41

Pen17zyme1B

GCTAATTGGTTAC TGTCAGCGACACGAA GTAACATGAGTTTTAC

44

Pen17zyme1C

CGCTAATTGGTTAGT CAGCTGACTCGAAC GTAACATGAGTTTTAC

45

Pen17zyme2

GAGTTTTACGTGTTA TGTCAGCGACACGAA CAATCGCTATGGTC

44

bioPEN17zyme1B

Biotin-GCTAATTGGTTAC TGTCAGCGACACGAA GTAACATGAGTTTTAC

44

bioPEN17zyme2

Biotin-GAGTTTTACGTGTTA TGTCAGCGACACGAA CAATCGCTATGGTC

44

bioantiPEN

Biotin-GAGTTTTACGAGTTACGTAACATGAGTTTTAC

32

  1. Underlined bold letters indicate residues that comprise the catalytic core of each DNAzyme
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BMC Biotechnology

ISSN: 1472-6750

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