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Table 1 Enrichment of phage displaying epitope by panning over mAb U001 from ph.D.-12 and Ph.D.-C7C

From: Mimotopes selected with a neutralizing antibody against urease B from Helicobacter pyloriinduce enzyme inhibitory antibodies in mice upon vaccination

Library

Cycle

coating mAb (mg/L)

Washing (TBST)

Input phage (pfu)

Output phage (pfu)

Recovery rate (%)

 

I

100

0.1%

1.5×1011

8.4×103

5.6×10-6

Ph.D.-12

II

10

0.3%

1.5×1011

8.0×104

5.3×10-5

 

III

1

0.5%

1.5×1011

3.0×105

2.0×10-4

 

I

100

0.1%

1.5×1011

7.6×103

5.4×10-6

Ph.D.-C7C

II

10

0.3%

1.5×1011

7.2×104

4.8×10-5

 

III

1

0.5%

1.5×1011

3.5×105

2.3×10-4

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BMC Biotechnology

ISSN: 1472-6750

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