Figure 6

Phage inhibition of mAb U001 binding to urease B protein. Wells of microtitre plates were coated with urease B protein and incubated with the mAb U001 (0.5 μg) plus increasing amounts (10⁹-10¹⁵ pfu) of the selected phage (clones D1 and H1). The binding was monitored by OD₄₉₂ measurements after reaction with the peroxidase-conjugated goat anti-mouse IgG. The inhibition ratio was determined by the following equation: [(activity without Ab - activity with Ab)/(activity without Ab)] × 100.