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Figure 2 | BMC Biotechnology

Figure 2

From: Recombinant Expression Screening of P. aeruginosaBacterial Inner Membrane Proteins

Figure 2

Example of the Western blots used to estimate relative expression levels. Proteins were expressed in strain BL21-AI at 37°C. Whole cell extracts were subjected to SDS-PAGE and transferred to membranes. The blots were probed with anti-6-His antibodies. Overall, approximately 65% of the proteins were expressed in at least one condition, and the sizes of most of the expressed proteins were found to correspond approximately with their predicted sizes. Lane 1 PA2628 (MW = 32 kDa), Lane 2 Control 6-His tagged protein (MW = 60 kDa), Lane 3 PA3773 (MW = 39 kDa), Lane 4 PA5291 (MW = 73 kDa), Lane 5 Molecular weight markers with weights given in kDA, Lane 6 PA4083 (MW = 26 kDa), Lane 7 PA4417 (MW = 51 kDa), Lane 8 PA5199 (MW = 49 kDa).

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