Skip to main content
Figure 5 | BMC Biotechnology

Figure 5

From: Improved Tet-responsive promoters with minimized background expression

Figure 5

Transduction of a Ptet-T6 regulated erbB2 expression unit into MDA-MB231.Ro cells. (A) γ-retroviral SIN-vector used to transfer erbB2 into rtTA-S2-positive MDA-MB231.Ro target cells. (B) FACS-analysis of transduced cell populations induced for the indicated time and Dox concentrations. The expression of the erbB2 transgene was detected by a monoclonal antibody (mAB ALX-804-573; Alexis) in combination with a fluorescence labeled secondary antibody (donkey anti mouse IgG-FITC, affipure, Jackson Immuno Res.). Mean fluorescence units (mfu, blot inset) are given for the complete population (≥ 92% purity). Clone #19 was derived from this population by limiting dilution.

Back to article page