Skip to main content

Table 1 Affinity of scFvE2/p17 for different variants of the conserved C-terminal epitope of HIV-1 MAp17 (a).

From: Baculovirus display of single chain antibody (scFv) using a novel signal peptide

Competitor Epitope Binding competition
vs p17.1 (%)
Origin or
HIV-1 isolate
Unrelated antigen
(negative control)
CD147 (M6) 5.2 ± 2.9 -
17.1
(positive control)
DTGHSSQVSQNY 89.5 ± 1.6 LAI (b)
17.3 DTGHSSQISQNY 74.7 ± 10.2 1M-1005 (c)
17.7 DTGHSSQASQNY 44.0 ± 18.0 g22s2 (d)
17.8 DTGHSKQVSQNY 81.5 ± 9.5 4 (e)
17.9 DTGNNSQVSQNY 68.8 ± 2.2 pNL4.3 (f)
Inverted p17.1 YNQSVQSSHGTD 3.8 ± 1.5 -
  1. (a) Competition for scFvE2/p17 binding between the dodecapeptide p17.1 (corresponding to residues 121-132 in the MAp17 from HIV-1LAI, used as immunogen) and other epitope variants was determined by ELISA, as examplified in Figure 4c. Data presented are m ± SEM (n = 3). Residues differing from the LAI prototype sequence are in bold.
  2. (b) [63].
  3. (c) [64].
  4. (d) [43].
  5. (e) [65].
  6. (f) [66].