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Figure 9 | BMC Biotechnology

Figure 9

From: Baculovirus display of single chain antibody (scFv) using a novel signal peptide

Figure 9

Functional analysis of BV-displayed chimeric scFv-N18E2/M6. The antigen-binding capacity and specificity of chimeric scFvE2/M6 displayed on the BV particle envelope was assessed by indirect ELISA, as described in a previous study [38, 39]. Aliquots of BV-N18E2/M6 and control BV-E2/p17 particles recovered from BV-infected Sf9 cells were added to CD147-BCCP-linked avidin-coated wells, and incubated for 1 h at RT. After washing steps, bound viral particles were detected by addition of anti-baculoviral envelope glycoprotein GP64 in TBS-BSA. Average of three separate experiments, m ± SEM. (**), P < 0.01.

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