Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA

BMC Biotechnology

Table 2 Production yield

TCR format	Total soluble production yield mg/L culture^a	Soluble monomeric production yield mg/L culture^b
4B2A1 scTCR Vαβ	3.6	2.1
4B2A1 scTCR Vβα	3.9	0.44
7A10B2 scTCR Vαβ	0.78	ND
7A10B2 scTCR Vβα	0.24	ND
1934^c	ND	3.8
c19^c	ND	3
2B4^c	ND	0.5
2B4^d	ND	0.015

^a Estimated periplasmic yield of total soluble scTCRs after IMAC purification.
^b Estimated periplasmic yield of monomeric soluble scTCR after size exclusion chromatography.
^c Values implemented from Maynard et al [9], scTCR-human C kappa fusion format
^d Values implemented from Maynard et al [9], scTCR format
ND; Not determined

ISSN: 1472-6750