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Table 1 Growth characteristicsa

From: Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA

TCR format

Vector

+IPTG

-IPTG

+Sucrose

+IPTG

+Sucrose

4B2A1 scTCR Vαβ

pHOG

0.6b

2.6

1.36

0.48b

 

pFKPEN

3

2.8

2.2

2.2

 

pFKPEI

2.8

2.8

2.1

2.4

4B2A1 scTCR Vβα

pHOG

2.6

2.6

2.2

2.2

 

pFKPEN

2.8

3

2.5

2.4

 

pFKPEI

2.7

2.8

2.2

2.2

7A10B2 scTCR Vαβ

pHOG

3

3

2.6

2.4

 

pFKPEN

0.8b

2.8

2.2

0.58b

 

pFKPEI

2.8

2.6

1.8

2

7A10B2 scTCR Vβα

pHOG

3

3

2.4

0.48b

 

pFKPEN

2.8

2.8

2.3

2.6

 

pFKPEI

2.8

2.8

2

2.2

XL1-Blue cells without plasmid

2.8

2.8

2

2.2

  1. a A600 nm after ON incubation of 5 ml expression cultures
  2. b No growth of cultures after induction
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BMC Biotechnology

ISSN: 1472-6750

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