Figure 4
From: Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA
Purity of proteins. (A) and (B); S75 size exclusion chromatography of scTCR 4B2A1 Vαβ and Vβα respectively. 50 μl of the IMAC purified material was subject to the purification procedure. In (A) fractions A14-B1 corresponds to the higher aggregates, while fraction B5-B7 corresponds to the monomeric scTCR 4B2A1 Vαβ, the monomeric fraction is highlighted with an arrow. In (B) fraction A15-B1 and B3-B4 corresponds to the higher aggregates, while fraction B6-B7 corresponds to the monomeric scTCR 4B2A1 Vβα, the monomeric fraction is highlighted with an arrow. (C) and (D); Western blots showing reduced and non-reduced scTCR Vαβ and Vβα after IMAC purification and S75 size exclusion chromatography. Samples of 0.25 μg were loaded on the gel and analysed with anti-His tag-HRP.