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Figure 2 | BMC Biotechnology

Figure 2

From: Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA

Figure 2

4B2A1 scTCR and rFkpA expression level. Western blot showing periplasmic expression levels of 4B2A1 scTCR variants and rFkpA from pHOG, pFKPEN (pEN) and pFKPEI (pEI) vectors under different expression conditions, detected with anti-His tag -HRP Ab and anti-Flag- M2 HRP Ab as described. The scTCR and rFkpA were developed with SuperSignal West Pico substrate and exposed to BioMax MR film for for 30 min and 30 sec, respectively, for the upper and lower panels. The expression analysis was repeated twice, and representative results are shown.

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