Figure 1
From: Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA
Schematic drawing of the scTCR molecules (left) and scTCR encoding vector constructs (right). (A) and (B) scTCR Vαβ and Vβα in pHOG. (C) and (D) scTCR Vαβ and scTCR Vβα in pFKPEN. (E) and (F) scTCR Vαβ and Vβα in pFKPEI. Abbreviations: lacPO, lac promoter, sd, Shine-Dalgarno sequence, T7g10, translation initiation region, t, T7 transcription terminator, xPO, native fkpA promotor, NcoI, NotI, NheI and ScaI, Restriction enzyme-sites.