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Figure 5 | BMC Biotechnology

Figure 5

From: Creation of a novel peptide with enhanced nuclear localization in prostate and pancreatic cancer cell lines

Figure 5

Glu-Oct6 facilitated entry of a PNA into DU-145 and PANC-1 cells. Glu-Oct6-PNA-FITC (0 to 1000 nM) was incubated with DU-145 or PANC-1 cells for 18 hr, at which time cells were harvested, washed, and processed for flow cytometry or imaging cytometry as described previously. Figure A: PANC-1 cells incubated with Glu-Oct6-PNA-FITC. IDEAS software (Amnis) was used to generate and colorize the images. Across each row of images, one is viewing the same cell under different fluorescence excitations. Thus for each cell type and peptide, the right-most column shows the merged images, the middle column shows the DRAQ5 images, and the left-most column shows the FITC images. Four thousand cells total were analyzed. Figure B: Uptake of Glu-Oct6-PNA-FITC by DU-145 cells quantified by flow cytometry. Figure C: Concentration-dependent increase in FITC fluorescence by PANC-1 cells incubated with Glu-Oct6-PNA-FITC, assessed by flow cytometry.

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