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Figure 5 | BMC Biotechnology

Figure 5

From: A miniaturized sandwich immunoassay platform for the detection of protein-protein interactions

Figure 5

Application of MSIP for verification of PPI candidates identified by YTH. Forty eight pairs of interaction candidates, listed in Additional file 1: Table S8 with lane numbers corresponding to their order in the table, were identified by YTH. HEK293 cells plated in 96-well dishes were cotransfected with FLAG-bait and myc-prey constructs or negative controls. Cell lysates were incubated in the frames of the anti-FLAG spotted slides (six slides were used), and protein-protein interactions were detected by monoclonal anti-myc-cy3 antibody (1:200). The slides were scanned by a fluorescence scanner, and the NFI of each PPI was calculated.

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