Figure 3

Probe based Real-Time PCR measures 1lox/2lox allele ratios with high accuracy. A, B, C Real-Time PCR analysis was conducted using standards with variable 1lox/2lox allele ratios. The resulting data were compared with expected ratios (C) and with ratios measured by capillary electrophoresis (CE), (B). As summarized in A the Real-Time PCR data closely reflected the expected data and the CE data. D: Deletion of Dnmt3a was measured in intestinal epithelial cells of mice homozygous for the Dnmt3a 2lox allele with Fabpl^{4× at -132}-Cre transgene (Cre⁺) and without Fabpl^{4× at -132}-Cre transgene (Cre^-). In agreement with previous studies using the same Fabpl^{4× at -132}-Cre strain, Cre⁺ mice showed low loop out efficiency in the proximal small intestine (1lox/2lox ratio of 0) and higher loop out efficiency in the distal small intestine (1lox/2lox approximately 0.5). Data from animal 1 middle and animal 2 middle are slightly out of standard range. As expected the 1lox allele was not detectable in Cre^- mice (mean = Cp of three replicates, SD = standard deviation).