Figure 4

Purification of Pf BCCP-79. PfBCCP-79 was coexpressed with BirA and BioB in B834(DE3) cells and purified by affinity chromatography. (A) Coomassie-stained SDS-PAGE gel showing fractions collected from each purification step. (B) Affinity blot using streptavidin-HRP to detect biotinylated proteins. Biotinylated PfBCCP-79 was present in the flow through from the metal chelate column and in the elution. The gel samples are labeled as follows: column input (CI); flow through from metal chelate column (FT1); 30 mM imidazole wash (W1); flow through from monomeric avidin column (FT2); buffer wash (W2); elution in 50 mM glycine pH 2.9 (E1); elution in 50 mM glycine pH 1.9 (E2).