Figure 5
From: Development of an EGFRvIII specific recombinant antibody
Detection of specificity of RAbDMvIII by Immunofluorescence. A) Immunofluorescence study shows that RAbDMvIII detects EGFRvIII protein in HC2 cells, B) Scrambled peptide competition shows detection of the EGFRvIII signal in HC2 cells by RAbDMvIII, C) Specific peptide (EGFRvIII) competition dampens the signal in HC2 cells, D) No signal is detected in A431 cells by RAbDMvIII, E) EGFR signal is detected in A431 cells by anti-EGFR antibody (sc-130, Santa Cruz).