Figure 4

Immunoprecipitation of EGFRvIII by RAb^DMvIII. Immunoprecipitation was performed with RAb^DMvIII, Monoclonal EGFRvIII antibody and RAb^CD133 (negative control for our recombinant antibody) on HC2 and A431 cell lysates. A) HC2 lysates immunoprecipitated by Monoclonal EGFRvIII antibody, RAb^DMvIII showed 145 kda band corresponding to EGFRvIII in the pellet whereas 145 kda band is seen in the sup of RAb^CD133 when immunoblotted with E3138 antibody from sigma. B) On the other hand A431 cell lysate showed 170 kda protein band corresponding to EGFR in the supernatant of Monoclonal EGFRvIII antibody, RAb^DMvII1 and RAb^CD133samples.