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Table 5 Phenotypical analysis of cells cultured in developed SFM and SCM.

From: Development of a serum-free medium for in vitro expansion of human cytotoxic T lymphocytes using a statistical design

Set #1

PBMCs (%)a

SFM (%)b

SCM (%)

CD14

5.21

-c

-

CD19

20.63

-

-

CD3

58.80

81.34

83.72

CD8

20.42

30.50

29.86

Set #2

PBMCs (%)

SFM (%)

SCM (%)

CD14

6.66

-

-

CD19

19.97

-

-

CD3

62.99

84.32

88.83

CD8

19.56

18.6

11.23

Set #3

PBMCs (%)

SFM (%)

SCM (%)

CD14

1.55

-

-

CD19

11.53

-

-

CD3

64.58

78.34

90.82

CD8

19.32

28.58

6.80

  1. a Freshly thawed PBMCs, before cultivation, were analyzed.
  2. b Cell samples in the three independent cultures were taken at a maximum viable cell concentration (refer to Figure 4B).
  3. c not detected.