Skip to main content

Table 5 Phenotypical analysis of cells cultured in developed SFM and SCM.

From: Development of a serum-free medium for in vitro expansion of human cytotoxic T lymphocytes using a statistical design

Set #1 PBMCs (%)a SFM (%)b SCM (%)
CD14 5.21 -c -
CD19 20.63 - -
CD3 58.80 81.34 83.72
CD8 20.42 30.50 29.86
Set #2 PBMCs (%) SFM (%) SCM (%)
CD14 6.66 - -
CD19 19.97 - -
CD3 62.99 84.32 88.83
CD8 19.56 18.6 11.23
Set #3 PBMCs (%) SFM (%) SCM (%)
CD14 1.55 - -
CD19 11.53 - -
CD3 64.58 78.34 90.82
CD8 19.32 28.58 6.80
  1. a Freshly thawed PBMCs, before cultivation, were analyzed.
  2. b Cell samples in the three independent cultures were taken at a maximum viable cell concentration (refer to Figure 4B).
  3. c not detected.