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Table 3 Maximum viable cell concentrations in 8 SFM designed by the fractional factorial method.

From: Development of a serum-free medium for in vitro expansion of human cytotoxic T lymphocytes using a statistical design

SFM

Maximum viable cell concentration (105 cells/ml)

 

Set #1

Set #2

Set #3

SFM #1

15.03

25.38

39.60

SFM #2

5.12

7.83

7.95

SFM #3

17.91

24.84

49.32

SFM #4

4.56

4.62

7.02

SFM #5

15.75

28.80

37.80

SFM #6

5.42

4.72

6.52

SFM #7

18.45

19.44

42.66

SFM #8

8.55

6.03

4.89

  1. PBMCs were obtained from three different donors (Set #1, Set #2, and Set #3). The seeding density was 5 × 105 cells/ml.
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BMC Biotechnology

ISSN: 1472-6750

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