Skip to main content

Table 2 Matrix of the fractional factorial design (24-1) for the 4 supplements.

From: Development of a serum-free medium for in vitro expansion of human cytotoxic T lymphocytes using a statistical design

  Basal SFM supplemented with:
SFM A B C D = ABC
  Phosphatidyl choline
(5 mg/l)
Polyamine supplement
(1×)
Antioxidant supplement
(1×)
Cholesterol
(4 mg/l)
#1 - - - -
#2 + - - +
#3 - + - +
#4 + + - -
#5 - - + +
#6 + - + -
#7 - + + -
#8 + + + +
  1. (-) no addition; (+) addition of the indicated amount of additives.