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Table 1 Composition of the basal SFM

From: Development of a serum-free medium for in vitro expansion of human cytotoxic T lymphocytes using a statistical design

RPMI1640 supplemented with:
Components Concentration (/l)
Bovine serum albumin 2.5 g
Insulin 5 mg
Ferric citrate 2 mg
Ethanolamine 1.22 mg
Linoleic acid 1 mg
Oleic acid 1 mg
Palmitic acid 1 mg
L-glutamine 584 mg
Sodium pyruvate 110 mg
2-mercaptoethanol 0.78 mg
1-thioglycerol 5.41 mg
RPMI1640 nonessential amino acids 20 ml
RPMI1640 vitamins solution 10 ml