Table 1 Composition of the basal SFM
| RPMI1640 supplemented with: | |
|---|---|
| Components | Concentration (/l) |
| Bovine serum albumin | 2.5 g |
| Insulin | 5 mg |
| Ferric citrate | 2 mg |
| Ethanolamine | 1.22 mg |
| Linoleic acid | 1 mg |
| Oleic acid | 1 mg |
| Palmitic acid | 1 mg |
| L-glutamine | 584 mg |
| Sodium pyruvate | 110 mg |
| 2-mercaptoethanol | 0.78 mg |
| 1-thioglycerol | 5.41 mg |
| RPMI1640 nonessential amino acids | 20 ml |
| RPMI1640 vitamins solution | 10 ml |
