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Figure 5 | BMC Biotechnology

Figure 5

From: Development of a serum-free medium for in vitro expansion of human cytotoxic T lymphocytes using a statistical design

Figure 5

Functional assays of CMV-specific CTLs generated from PBMCs cultured in the developed SFM and SCM. A. Cytotoxicity of CMV-specific CTLs generated from PBMCs cultured in the developed SFM (black circles) and SCM (white circles) was tested for their ability to lyse target cells. B. CMV-specific responses obtained by ELISpot using the developed SFM and SCM as culture media. Functional assays were duplicated independently and each experiment was carried out in triplicates. Error bar represents the standard deviation (n = 3).

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