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Figure 1 | BMC Biotechnology

Figure 1

From: Heterologous expression of leader-less pga gene in Pichia pastoris: intracellular production of prokaryotic enzyme

Figure 1

The effect of transient phase profile on hPGA Ec production in fed-batch cultures of the strain P. pastoris X-33(pPIC-PA1). Time courses of the values of the cell dry weight (g l-1), optical density (OD600), volumetric activity (U l-1) and specific activity of hPGAEc (U gcdw-1) assayed in the cultures in a 10- l stirred bioreactor. Phases of the cultures: GB, glycerol batch culture; GF, glycerol feed culture; TC, transient phase with linear decrease of glycerol concentration to 0 and constant feed of methanol (A- final concentration of methanol 0.5%, B- final concentration of methanol 1.5%); SI, transient phase with starvation for glycerol and single dose of methanol at the end of the phase (C- final concentration of methanol 1.5%, D- final concentration of methanol 0.5%); MF, methanol feed culture.

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