Baculovirus productivity. Monolayers of UFL-Ag-286 cells were treated during 48 h both in standard condition (10% FBS, black) or synchronized condition (1% FBS, gray) in GRACE's medium, then infected with AgMNPV-SF using three different MOIs (0.1, 1, 10) (n = 3 for each assay, * P < 0.05, ** P < 0.01, *** P < 0.001, Student's T test); and maintained in GRACE's medium with 10% FBS. A. BVs isolated from supernatants and quantified by plaque assays. B. OBs isolated from infected cells and quantified by microscopic observation. Error bars are the corresponding standard deviations.