Figure 5

Transcript analysis of ie1 , gp64 and polh genes of AgMNPV. Monolayers of UFL-Ag-286 cells were treated during 48 h both in standard condition (10% FBS) and synchronized condition (1% FBS) in GRACE's medium, then infected with AgMNPV-SF using three different MOIs (0.1, 1, 10) (n = 3 for each assay); and maintained in GRACE's medium with 10% FBS. After this, cells were collected at 1, 12 and 24 hours to isolate whole RNA, and later hybridized with ie1, gp64, polh (AgMNPV genes) and actin (UFL-Ag-286 gene) probes in independent assays by Slot Blot strategy. The different detected signals for each viral transcript were quantified by image densitometry, relativized using the actin mRNA level and considering as 100% the highest value reached in each test. The error bars are the corresponding standard deviations.