Genetic transformation of lignin degrading fungi facilitated by Agrobacterium tumefaciens

BMC Biotechnology

Table 1 Transformation efficiencies (%) in different fungal species at varying co-cultivation temperatures

Basidomycetous Fungi	Percent transformation efficiency (η)* at different Co-cultivation temperature
	20°C	23°C	26°C	29°C
Ganoderma sp. RCKK-02	65	48	32	17
P. cinnabarinus	65	45	30	15
Crinipellis sp. RCK-1	75	65	48	ND
Pleurotus sojur-caju	50	34	45	ND
P. chrysosporium	75	63	34	20
Fungal isolate BHR-UDSC	50	30	18	ND

ND- Not Detected
* $η = \frac{no . of transformed pellet positive for GUS}{no . of wild type fungal pellets} \times 100$

ISSN: 1472-6750