Figure 5
From: Genetic transformation of lignin degrading fungi facilitated by Agrobacterium tumefaciens
Molecular screening of transformants. (a). PCR of transformed mycelia using GUS-GFP fusion primer. Lane M: DNA molecular weight marker (kb); Lane 1&2: Ganoderma sp. RCKK-02; Lane 3&4: P. cinnabarinus; Lane 5&6: Crinipellis sp. RCK-1; Lane 7&8: P. sojur-caju; Lane 9&10: P. chrysosporium; Lane 11&12: Fungal isolate BHR-UDSC. Lanes 2,4,6,8,10 & 12 are acetosyringone (AS) pre-induced, whereas others are without AS. The Lane C1 & Lane C2 were positive control and negative control, respectively. (b). Southern blot analysis of transformed fungus using radiolabelled hpt gene probe. Lane WT: Untransformed; Lane A: Ganoderma sp. RCKK-02; Lane B: P. cinnabarinus; Lane C: Crinipellis sp. RCK-1; Lane D: P. sojur-caju; Lane E: Positive control; Lane F: P. chrysosporium; Lane G: Fungal isolate BHR-UDSC. (c). Southern blot analysis of different transformants using KanR probe. Lane C: Untransformed control; Lane 1: Ganoderma sp. RCKK-02; Lane 2: P. cinnabarinus; Lane 3: Crinipellis sp. RCK-1; Lane 4: P. sojur-caju; Lane 5: P. chrysosporium; Lane 6: Fungal isolate BHR-UDSC.