Figure 8

Immunoblot analysis of growth factor binding to Pln.198 and Pln.247. 500 ng growth factors were bound to nitrocellulose, the blots were blocked, and then incubated in either 1 ug/ml Pln.198 or Pln.247 (panel A) or rhPln.247 pre-digested with heparinase III (panel B). Detection was performed with anti-perlecan domain 1 primary antibody CSI 001-71. Note that while both the background BSA signal and the internal control rhPln.247 signals are slightly higher in panel B (bottom two samples), a decrease in the growth factor binding to Pln.247 was present after digestion.