Figure 7From: Similarity of Recombinant Human Perlecan Domain 1 by Alternative Expression Systems Bioactive Heterogenous Recombinant Human Perlecan D1Growth-factor binding to rhPln.198, rhPln.247, and HSPG in solid phase. Commercial HSPG, Pln.247, or rhPln.198 were bound directly to polystyrene microtiter wells. The proteoglycan samples were incubated with 6-fold dilutions of rhFGF-2. After washing away unbound FGF, the bound FGF was detected with anti-FGF biotin conjugates.Back to article page